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The first extracellular loop is notably larger than the second and contains charged amino acids that determine the proteins charge selectivity and barrier properties

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The supernatant was collected, dried under nitrogen gas, and resuspended in 100 L of 80% HPLC grade methanol in HPLC grade water for subsequent analysis by mass-spectrometry

This is consistent with another study which showed that Tanshinone IIA (Tan) inhibit atherosclerotic plaque formation in ApoE -/- mice and it is suggested that Tan regulates cholesterol metabolism by reducing the expression of SR-A, while further enhancing ABCA1 and ABCG1 expression in human THP-1 cells via activation of ERK/Nrf2/HO-1 pathway (Liu et al., 2014)

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