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Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)

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149 Results from several meta-analyses identified that there are groups with an increased risk of B12 deficiency, namely those with pre-existing moderately low vitamin B12 levels 71 or those aged 60 years, and/or on 2000 mg/day metformin and/or 3 years of treatment

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Key nutrients for menopausal health include: Calcium and Vitamin D : These are crucial for maintaining bone density and preventing osteoporosis