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The PK parameters of r-hGH were calculated according to non-compartmental standard methods using the validated computer program KINETICA (Version 4.4.1, Thermo, Philadelphia, USA)

In contrary, on 1 day treatment groups, there was no significant difference in protein expression in both doses of mitragynine treatments

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Principal component analysis (PCA) was performed using Sirius version 10.0 (Pattern Recognition Systems AS, Bergen, Norway)