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Morris ZS, Demaria S, Monjazeb AM, Formenti SC, Weichselbaum RR, Welsh J, Enderling H, Schoenfeld JD, Brody JD, McGee HM, Mondini M, Kent MS, Young KH, Galluzzi L, Karam SD, Theelen WSME, Chang JY , Huynh MA, Daib A, Pitroda S, Chung C, Serre R, Grassberger C, Deng J, Sodji QH, Nguyen AT, Patel RB, Krebs S, Kalbasi A, Kerr C, Vanpouille-Box C, Vick L, Aguilera TA, Ong IM, Herrera F, Menon H, Smart D, Ahmed J, Gartrell RD, Roland CL, Fekrmandi F, Chakraborty B, Bent EH, Berg TJ, Hutson A, Khleif S, Sikora AG, Fong L

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Surprisingly, our study found that ethanol upregulated GPx4 protein levels both in vitro and in vivo

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The membrane was washed with PBS containing 0.1% Tween-20 three times for 10 min, and chemiluminescence was used to detect the antibody-labeled proteins using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, Inc.) and detected with the LAS-3000 Luminescent Image Analyzer (Fujifilm Holdings Corporation, Tokyo, Japan)

10.1016/j.jep.2014.07.037 56 LawrenceN