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SPS involves three severe stressors (a 2 h restraint stress, followed by forced swimming for 20 min, and finally exposure of the animal to ether until loss of consciousness)

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These effects collectively contribute to a reduction in key renal function indicators, such as serum creatinine (SCr), blood urea nitrogen (BUN), and 24 h urinary protein, by 2041.1% compared to baseline levels, as well as amelioration of renal histopathological alterations, thus effectively preserving renal function and retarding DN progression (7275) (Figure 5)

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