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They were then spun down at 15,000 g for 45 minutes at 4C and the supernatant was passed over a nickel resin column (Thermo Fisher Scientific, # PI88222) and eluted using buffer B (30mM K2HPO4, 300mM NaCl, 500mM Imidazole, pH 8)

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Serum total antioxidant status, erythrocyte superoxide dismutase and whole-blood glutathione peroxidase activities in the stanislas cohort: Influencing factors and reference intervals

The GC column was 5% phenyl and the temperature ramp ranged from 40 to 300 C in a 16 minute period

In recent years, it has become clear that, although free thiols are found in almost all new cultivars that stand out due to their fruity aromas, precursor structures containing bound thiols are present in high concentrations in every hop variety