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doi: 10.1016/s0016-5085(96)70055-0

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Fixed tissues were incubated in 10% sucrose in 1 PBS for 30 min and then incubated in 20% sucrose in 1 PBS at 4 C for 4 h, followed by embedding in OCT mounting compound (TissueTek, Sakura) on dry ice and stored at 80 C

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