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Lancet 370 , 14221431 (2007)

To induce GPX2 overexpression, cells were treated with 1 g/mL doxycycline for 24 h

(Hybertson et al

The expression of adipogenic genes is decreased in obesity and diabetes mellitus

These mice were perfused with PBS at 0.5, 2, 6, 12, 24 h after the last treatment ( n = 3 per group), and their brains were collected and homogenized in the buffer containing 10 mM HEPES, 1.5 mM MgCl 2 , 10 mM KCl, 100 mM DTT, and 1 mM PMSF (pH 7.9) (5 ml/gram of brain) by sonication on ice (one pulse of 0.75 s for 2 min, UP200H, Hielscher, USA)

Proteins were precipitated by adding four volumes of ice-cold acetone and subsequent centrifugation (21.000 g , 20 min, 4 C)