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On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin

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Seventy-seven percent had been diagnosed with diabetes for over ten years

It is not naturally found in plant-based foods, which is why vegans and vegetarians may be at risk of developing a deficiency if they do not consume enough fortified foods or supplements

Systemic delivery of siRNA specific for silencing TLR4 gene expression reduces diabetic cardiomyopathy in a mouse model of streptozotocin-induced type 1 diabetes

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