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Primary neuron cultures In preparation for primary neuron harvests, glass-bottom chamber slides were coated with 0.5 mg/mL poly-D-lysine (Sigma, P7886-100MG) in borate buffer [12.5 mM sodium borate decahydrate (VWR, MK745706) and 50 mM boric acid (Sigma, B6768-1KG)] overnight at room temperature, then washed 4x with sterile deionized water

it is a very potent vasoconstrictor and cardiac stimulant

J Neurosci 13:623631 El Hindy M, Hezwani M, Corry D, Hull J, El Amraoui F, Harris M, Lee C, Forshaw T, Wilson A, Mansbridge A, Hassler M, Patel VB, Kehoe PG, Love S, Conway ME (2014) The branched-chain aminotransferase proteins: novel redox chaperones for protein disulfide isomeraseimplications in Alzheimer's disease

Grade I : A mild shoulder separation involves a sprain of the AC ligaments that does not move the collarbone and looks normal on X-rays

To get the full "repair" benefits, aim for 79 hours of quality rest

doi: 10.1016/j.snb.2019.126646