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A constant amount of plasmid cDNA (2.5 g) was transfected into the HEK-293T cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturers instructions

On the other hand, studies also revealed that selective iNOS inhibitors could impair hepatic blood flow and increase liver injury, suggesting that iNOS, in a way similar to eNOS, exerts an essential role in maintaining liver sinusoidal perfusion in LIR (570, 1347)

Therapeutically, traditional dopaminergic treatments remain effective for motor symptoms but offer limited longterm disease control

FIGURE 3 3.4 Role of other micronutrients in shaping amyloid fibrillation: iodine and selenium in regulating -synuclein fibrillation Apart from zinc, other physiologically significant non-metal micronutrients such as iodine and selenium have also been demonstrated to possess anti-amyloidogenic properties

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