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Briefly, cells were washed twice with PBS, fixed with 10% formalin for 30 min at room temperature, and stained with oil red O working solution ( 0.2% oil red O in 99% isopropanol) for 30 min

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After the treatment, the area will feel similar to light sunburn and the pigmented lesions will get darker and sometimes red

The researchers noted a clear dose-response relationship, where higher doses yielded better clinical outcomes, particularly in patients who carried variations in the MTHFR gene

Lancet 1987;2:740 4