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To account for differences in library size, gene-level expression data were normalized using the Trimmed Mean of M-values (TMM) method implemented in edgeR 138

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Its a critical decision based on specific research objectives

The Ulp1-treated samples were incubated with Ni-NTA resin to remove the His14-SUMO tag, and the unbound proteins were concentrated and loaded onto a Superdex 200 Increase column equilibrated with the SEC buffer

In GST of both subgroups and in mitochondrial GST, these amino acid residues essential for GSH activation are located in the so-called catalytic loop that is next to the first -sheet in the thioredoxin-like domain

After intraarticular injection, thermoresponsive hydrogels quickly gel at local lesion temperature and provide sustained delivery of mitochondria-targeted agents