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Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues

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According to the results of our pilot experiments (data not shown), the HSA concentration, the ratio of GSH to HSA, the temperature of the reduction reaction, the ratio of ATRA to HSA, the ratio of ATO and ATRA, the injection speed of ATRA solution, and the volume ratio of ethanol and water were set to 4%, 38.5%, 40 , 3%, 12.7%, 1.5 mL min 1 , and 5:1, respectively

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Keep the metered spray pump clean and refrigerated at all times when not in use to minimize degradation of the peptide