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Both total glutathione and GSSG determinations are based on the reaction where GSH-dependent conversion of a GSH probe, Luciferin-NT, to luciferin by a glutathione-S-transferase enzyme is coupled to a firefly luciferase reaction

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After washing, the cells were further incubated with DAPI staining solution for 20 min at 37C

Metabolism and Biochemistry Sulfur metabolism under stress: Oxidized glutathione inhibits methionine biosynthesis by destabilizing the enzyme cystathionine -synthase 1

Although the time course of Nrf2 stabilization and nuclear translocation indicated a rapid increase with a peak at approximately 6 h for DEM, and a slow but gradual rise triggered by NFT, maximal accumulation of Nrf2 protein, as well as the induction of GCLC and glutathione synthesis, were almost identical for both NFT and DEM after incubation periods of and beyond 24 h (Fig