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In addition, the vaccine improved the physical activity of middle-aged mice (50 weeks old) and extended the median lifespan of ZMPSTE24 KO mouse (model of progeria), an extreme model of accelerated aging 62

Pain behavior was quantified periodically at 5- and 35- min intervals (representative values of phases 1 and 2) by counting the number of flinches exhibited by the injected paw after injection