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You want localized, sustained dermal concentration, not a plasma spike that clears before fibroblasts finish one cell cycle

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Most existing studies are limited to in vitro or animal models, and data on clinical isolates or patient outcomes are scarce

Then the TEM-1 beta -lactamase gene was fused with the binding domain of pGBT9 (+2) to generate bait plasmid, pYG111, the structure of which was tested by digestion with restriction endonucleases Eco RI and Bam HI (Fig