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The protocol for animal care and usage was approved by the Animal Care and Use Committee of Nanjing University of Chinese Medicine (Approval number: A210101) and followed the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health

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After centrifugation, samples were measured by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)

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Caffeine helps increase alertness and boosts the bodys metabolic rate to burn more calories

Additionally, it may help reduce muscle soreness and fatigue after exercise, allowing for faster recovery