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The choice of cell type, culture conditions, and endpoint all affect the result

Three-dimensional culture of annulus fibrosus cells within PDLLA/Bioglass composite foam scaffolds: assessment of cell attachment, proliferation and extracellular matrix production

The lack of these receptors ensures GI macrophages are unable to respond to ligands that would typically activate other immune cell types thereby preventing excessive IRs (Brenchley and Douek 2012)

These findings suggest that the RGMa negatively regulates hippocampal neurogenesis and is involved in diabetes mellitus-induced cognitive decline

There are also rarer risks

Just as any substance affecting hormone production carries theoretical risks of imbalance, excessive kisspeptin signaling could potentially produce unwanted effects