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Department of Health and Human Services, Food and Drug Administration (2018) Bioanalytical method validation guidance for industry

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The cells were maintained at 37C in a humidified atmosphere with 5% CO 2 and were subcultured two to three times a week

RSC Adv 7:3250832517 Cheng C-Y, Barro L, Tsai S-T, Feng T-W, Wu X-Y, Chao C-W, Yu R-S, Chin T-Y, Hsieh MF (2021) Epigallocatechin-3-gallate-loaded liposomes favor anti-inflammation of microglia cells and promote neuroprotection