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The secondary antibodies included Cy3 goat anti-rabbit IgG (1:50 dilution, GB21303, Servicebio, China), Alexa Fluor 488 goat anti-rabbit IgG (1:200 dilution, ab150077, Abcam, America), Alexa Fluor 488 goat anti-rat IgG (1:200 dilution, ab150165, Abcam, America), Alexa Fluor 647 donkey anti-rabbit IgG (1:200 dilution, ab150075, Abcam, America), and Alexa Fluor 647 goat anti-mouse IgG (ab150115, Abcam, America, 1:200 dilution)