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While intravenous or intraperitoneal routes may improve bioavailability, they are difficult to apply in small animal models

The systematic review protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO), registration number: CRD42024554513 (Available from) ( 2.2 PICOs question The primary research question addressed in this review was: What is the effectiveness of sublingual/oral administration of Vitamin B12 alone or compared to other forms of administration (e.g., intramuscular injection)?

Intramuscular Vitamin B-12 Injections Also called shots, intramuscular vitamin B12 injections are a common and effective way to treat a deficiency

After being fed HFD for a total of 16-weeks (an appropriate rodent model of DIO and comparable to human obesity [21]) and reaching pre-arrival body weights (~38g), mice were randomized into balanced control and treatment cohorts (n=9/cohort), with similar group average body weight and standard deviation

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Proper technique involves cleaning the injection site with alcohol, pinching the skin to create a fold, inserting the needle at a 45-90 degree angle, injecting slowly, and rotating injection sites to prevent irritation