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Contrarily, we recently demonstrated that the new initial target is the tongue, resulting in spontaneous reversal of strong alcohol-stomach lesions

Purification of DYKDDDDK-tagged Proteins Expressed in Mammalian Cells This protocol depicts an efficient method to obtain high yields for purification of up to 10 mg of DYKDDDDK-tagged recombinant protein/ antibody from ~200 to ~900 ml tissue culture supernatant

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TRPM7 D1054A, an acid-activation-deficient mutant, abolished acidification-evoked cell volume increase and cell death, suggesting acid-evoked activation of TRPM7 leads to a necrotic volume increase (NVI) followed by necrotic cell death