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Biomaterials science and engineering

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Birchmeier, C

23 Accordingly, reduced expression of PD-1 on HBV-specific CD8 + T cells with high levels of T-bet was found to support the key role for T-bet in regulating inhibitory molecules of exhausted T cells during chronic HBV infection, 36 which may account for T-bet deficiency as an important mechanism behind chronic infection

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CD8 + T cells were activated for 5 days with magnetic Dynabeads (Invitrogen) bound with anti-CD3 (OKT3, BioLegend) and anti-CD28 (CD28.2, BioLegend) antibodies in the presence of IL-2 (100 U/mL)