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For those using nasal spray formulations, the typical dosage is 30mg per spray, administered once daily

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10 L of each serial dilution is then added to 90 L of blank plasma for final concentrations of 0.01, 0.02, 0.05, 0.1, 0.2, 1, 10, 20, 50, and 100 g/mL

Nat Cell Biol 8:10641073 Brenkman AB, de Keizer PL, van den Broek NJ, Jochemsen AG, Burgering BM (2008) Mdm2 induces mono-ubiquitination of FOXO4