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Intestinal changes were evaluated through 1) gut microbiota profiling and metabolite production (short-chain fatty acids (SCFAs), indoxyl sulfate), 2) structural and barrier integrity via histology, mucus staining, and tight junction markers (ZO-1, occludin, claudin-5), 3) mucosal immunity through M1/M2 macrophage profiling and Th17/Treg ratios, and 4) expression of inflammatory and oxidative stress markers

Although a complex network of pathways, numerous kinases, and many phosphorylation sites are involved in eNOS activity, the regulation and phosphorylation of Ser1177 is a critical requirement for this process [28]

Ueno, Yuki, et al

poor storage accelerates degradation

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