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Additionally, MTX has demonstrated efficacy when paired with anti-tumor necrosis factor-alpha (TNF-) drugs in the treatment of individuals suffering from ulcerative colitis, breast cancer, lung carcinoma, head and neck malignancies, and ovarian carcinoma (Chande et al

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The supernatant was collected to measure uricase concentration using BCA Protein Assay Kits

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Biocatal Agric Biotechnol 27:101677