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Protein bands were visualized using an ECL substrate (34580, Thermo Scientific) and detected using the ChemiDoc XRS+ Imaging System (Bio-Rad)

Calcitriol can affect neuroplasticity, apoptosis, and gene expression (such as neurotransmitters, neurotrophic, and synaptic proteins) (102)

The resulting chain reaction leads to the peroxidation of the mitochondrial and plasma membranes

It applies an ascending course that gradually affects the midbrain, especially the substantia nigra, and spreads from there to impair the mesocortex and neocortex

1:500 (v/v) added to the methanol and used for standardization of the metabolite intensity and to adjust the migration time), and were scraped from the dish