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The kinetics of the formation of TNB (5-thio-2-nitrobenzoic acid) were immediately recorded at 412 nm, every 30 s over a 2-min period

This is significant as an enzyme usually does not change the equilibrium of the reaction, indicating that PIN1 preferentially catalyzes the trans to cis isomerization of pS163-P motif in CDH1, which may lead to increase of phosphorylated CDH1 because CDH1-specific phosphatase does not engage with cis proline 46

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In this era, the movement of people across the Australasian region helped establish maritime trade and communication networks in the Southeast Asia region (Solheim et al., 2006)