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Cells were treated with ENDOs or vehicle and TLR9 activation in response to CpG (5 M) was analyzed by measuring SEAP activity, monitored using QUANTI-Blue (InvivoGene) following the manufacturers recommendations

In AS, oxidative stress first causes endothelial dysfunction, thus increasing vascular permeability 24

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Vagal Tone Support: By providing the raw materials for acetylcholine synthesis, the formula supports the overall health and signaling capacity of the parasympathetic nervous system