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Afatinib (0-10000 nM) effectively inhibits anchorage-independent proliferation of NIH-3T3 cells ectopically expressing EGFR mutants, and inhibits cell proliferation of H1666, H3255, and NCI 1975 cells [1]

Circ Res 100:174190

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Alongside IL-1, MSU crystals also induce macrophages to produce caspase-11, a module that serves both as a recruiting agent for circulating neutrophils and a stimulatory cytokine prompting neutrophils to form NETs

Then, there are special situations where first-line treatment may not be successful, as seen in difficult-to-treat AIH (DTT-AIH), AIH associated with cirrhosis and overlap syndrome