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After centrifugation at 18,000 rpm for 30 min, the supernatant was collected and incubated with glutathione-Sepharose 4B at 4 C, followed by washing with PBS buffer

SFN induces a pro-oxidative milieu in untransformed human T-cells ROS are ubiquitously generated and recognized as important signaling molecules for T-cell activation, but excessive ROS generation or prolonged exposure to high ROS concentrations impair T-cell functions ( 2 DCFDA and treated with SFN prior to flow cytometric analysis

Eriau, E., Paillet, J., Kroemer, G

The total radical-trapping antioxidant parameter (TRAP) assay quantifies the capacity of antioxidants to delay fluorescence decay in a probe (such as R-phycoerythrin or dichlorofluorescein) under controlled peroxidative conditions initiated by AAPH-generated ROO

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