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Serum IgG and mucosal secretory IgA (sIgA) were detected with HRP-conjugated goat anti-mouse IgG and HRP-conjugated goat anti-mouse IgA (Jackson ImmunoResearch, USA), respectively

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A clerk in the ship chandlery and grocery business, he moved to New York City in 1847 where he joined the Freemasons Star of Bethlehem Lodge in 1854 and started a shipping and commission business that same year

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