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For western blot analysis samples were separated by SDS-PAGE and transferred to 0.45 m nitrocellulose membranes

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The m6 A modification is dynamically regulated by writers (installers), erasers(removers), and readers(recognizers), such as YT521-B homology (YTH) family proteins (YTHDF1/2/3 and YTHDC1/2) and insulin-like growth factor 2 mRNA-binding proteins (IGF2BP1/2/3) (Zaccara et al