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Based on an increasing understanding of the complexity of regulating host cell necrosis in COVID-19 and the connection with the intrinsic immune defense mechanism of SARS-CoV-2 infection (innate immunity of cells), we speculate that regulated necrosis plays a double-edged sword role in COVID-19 and seems to depend on many factors, including downstream effector molecules (various cytokines and chemokines), the specific cell death pathway involved, and the immune response at different clinical stages of COVID-19 progression

*Correspondence: Oriana Simonetti, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Kwamman, Y., Mahisanunt, B., Matsukawa, S

Western blot HepG2 cells were treated for 24 or 48 h with selected drugs and were harvested in the following buffer: 150 mM NaCl, 1% Igepal, 50 mM TrisHCl pH 7.4 with Protease inhibitor cocktail (Roche)

doi: 10.1016/j.celrep.2017.12.077 85 ShimadaKHayanoMPaganoNCStockwellBR