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Microbially derived indoles (indole, indole-3-acetic acid/IAA, indole-3-propionic acid/IPA, indole-3-lactic acid/ILA) critically influence CRC progression: ILAthe most extensively studied anticancer indoleactivates CD8 + T cells by enhancing dendritic cell IL-12 production via histone modification and chromatin accessibility (316), promotes effector molecule release by inhibiting the cholesterol metabolism gene Saa3 in CD8 + T cells (316), and reduces CD86 + proinflammatory macrophages via AhR/p-AKT/IL-1 signaling (374)

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Upregulation of GST genes can be the consequence of such oxidation, which is also in line with the upregulation of ergosterol biosynthesis genes (Additional file 2) and the elevated sterol content of NaNO 2 treated cells (Fig