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Description

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Necroptosis regulates PH mainly through non-classical regulatory pathways, which may be a protective mechanism after apoptosis is inhibited

The supernatants were collected by centrifugation at 5000 rpm for 5 min at 4 C and precleared by incubated with GFP-Trap agarose beads (Chromotek) or HA-Beads (Roche) for 2 h at 4 C with rotation