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These include constrained tissue sizes, difficulty in cell retrieval for downstream analysis, and, most critically, a limited volume of effluent for comprehensive molecular profiling (e.g., proteomics, metabolomics), which is essential for biomarker discovery and mechanistic studies (215218)

[DOI] [PubMed] [Google Scholar] 137.Gan J., Ruan Q., He B., Zhu M., Shyu W.C., Humphreys W.G

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Protein mislocalization and cytoplasmic aggregation are prominent features in both genetic and nongenetic ALS, leading to loss of essential cellular functions