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The viral genome The SARS-CoV-2 genome is a 29.9-kb RNA that consists of 14 open reading frames (ORFs) encoding two large polyproteins (ORF1a and ORF1b) and 13 small ORFs that encode viral structural proteins and other polypeptides

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After centrifugation, methanol-containing proteins were precipitated with cold trichloroacetic acid (TCA) and reduced with 500 mM sodium borohydride in 0.2 M borate buffer (pH 9.2) after overnight incubation at room temperature

Int J Cancer 149(7):14951511 Ye Z, Hu Q, Zhuo Q, Zhu Y, Fan G, Liu M et al (2020) Abrogation of ARF6 promotes RSL3-induced ferroptosis and mitigates gemcitabine resistance in pancreatic cancer cells