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Five blood spots, comprising approximately 150250 L per spot, will be collected using mini contact-activated lancets (BD 366594 Microtainer, BD Biosciences, San Jose, CA, United States) to prick the finger after the participant runs their non-dominant hand under hot water for 2 min

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Additionally, since serum GPX-3 concentration was inversely correlated with BVAS, we divided the study participants into two groups according to each of the nine systemic items of BVAS and compared serum GPX-3 concentrations between the two groups (Mukhtyar et al., 2009)

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This may contribute to a reduction in the risk of chronic diseases