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Patients over the age of 18 were excluded from the study, leading to the exclusion of 76 (24.3%) patients from the study

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Total RNA was isolated from the cells using the easy-BLUE Total RNA Extraction kit (Intron Biotechnology Inc., Seongnam, Korea) and template RNA was amplified using 1 l RT reaction buffer, along with primers (Bioneer Corporation, Daejeon, Korea), dNTPs, and 0.5 U Taq DNA polymerase (Invitrogen Life Technologies, Carlsbad, CA, USA), in a final volume of 25 l

Neuromodulation of bone: role of different peptides and their interactions (Review)

Science 299 , 117120 (2003)