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RNA ISH was performed as previously described ( Nuclear and Cytoplasmic RNA Fractionation Analysis Nuclear and cytoplasmic RNA isolation in the mle-12 cells was performed using the Cytoplasmic and Nuclear RNA Purification Kit (Norgen, Belmont, CA, United States) according to the manufacturers instruction

Leucine is an essential amino acid

These biomarkers not only act as indirect indicators of resolution potential but also provide tools to evaluate disease progression, predict therapeutic responses, and guide indication selection and target identification for PRLM-based therapies [29]

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In the biotechnology industry, a secondary enzyme such as formate dehydrogenase has been explored for regenerating NADH from NAD + that will provide a reduced cofactor for reductase/dehydrogenase reactions (Hummel and Grger, 2014)