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Transwell chambers were assayed for cell invasion ability by applying 100 L of DMEM-diluted Matrigel gel (3:7 dilution) to the chambers to be checked and incubated at 37 C for 30 min to form a gel, then pre-treated MIA PaCa-2 and PANC-1 cells were resuspended with DMEM and spread in small chambers at 50000/well, and the lower chamber was placed in 20% FBS DMEM, incubated at 37C 5% CO 2 for 48 hours

Alvarez-Suarez JM, Giampieri F, Tulipani S, Casoli T, Di Stefano G, Gonzlez-Params AM, Santos-Buelga C, Busco F, Quiles JL, Cordero MD, Bompadre S, Mezzetti B, Battino M (2014) One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress markers and platelet activation in humans

To determine whether NRF2 can localize to the Ptgir gene, we first analyzed NRF2 chromatin immunoprecipitation sequencing (ChIPseq) data from Keap1 / mouse embryonic fibroblasts (MEFs) that display constitutive NRF2 activity 31

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A small randomised study found that nebulised glutathione caused substantial bronchoconstriction in participants with mild asthma