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2.2 Experimental animals A total of 80 specific pathogen free (SPF) Kunming mice (40 male and 40 female) with an average body weight of 20 2 g were purchased from Liaoning Changsheng Agricultural Science and Technology Co., Ltd

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TMAO regulates APOA4, PCSK9 expressions, and cholesterol metabolism in vitro PCSK9, HMGCR, ABCG5 , and ABCG8 mRNA levels were significantly elevated (Fig

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