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We became interested in this compound as the ethylene glycol group not only masks the indole, which is particularly relevant in this study, but also dramatically alters the shape of the entire alkaloid scaffold

Notably, translational delivery is optimized by ex vivo pretreatment during CAR-T cell manufacturing (2-day exposure during activation) rather than systemic administration: in vivo pentanoate fails to enhance anti-PD-1 therapy, whereas ex vivo programming preserves its metabolic-epigenetic effects and avoids off-target immunosuppression (e.g., Treg induction, which is triggered by butyrate but not pentanoate) (108, 109)