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In this study, we investigated the time-dependent decrease in reducing activity of DTT during prolonged protein synthesis using the PURE system

doi: 10.1007/s10557-005-6892-4 84 QaatyNWangYWangAMaoSVincentMHusainMet al

Levels of pY were normalized to total IGF1R levels and shown as intensities relative to that of IGF1R WT in 50 nM IGF1 treated cells

,,AnschauV,Ferrer-SuetaG,Aleixo-SilvaRL,Bannitz FernandesR,TairumCATonoliCCCet al.Reduction of sulfenic acids by ascorbate in proteins, connecting thiol-dependent to alternative redox pathways.2020;156:207216 15

It is also a co-factor for GPX4-mediated lipid detoxification 35 , and GPX4 activity is necessary for the survival of several tissues, including the liver 36 and kidney 37