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Results Manipulation check To monitor the levels of T and other hormones that might influence decision making during the experiment (e.g., cortisol) 43 , participants provided one pre-treatment saliva sample (that also allowed us to investigate the correlation between behavior and basal T) and three post-treatment saliva samples, that were assayed by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Cureus 14 , e32540 (2022)

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Plasmid transfection was performed with a mixture of 400 ng Gio-LgBiT, 500 ng G-SmBiT, 500 ng G, 600 ng ghrelin receptor by Lipofectamine 2000 (ThermoFisher Scientific) in 200 L of Opti-MEM (Gibco)

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