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The eluted proteins were buffer-exchanged to DPBS and concentrated using Amicon Ultra-15 Centrifugation Filter Units (Millipore) with a 30 kDa cutoff by centrifugation at (290400) g at 4 C

so when taken as dietary supplements, the three need to be in balance

Moisturizer can be applied afterward if needed for dryness

J Clin Invest 89 : 19081913 Ethics declarations Competing interests The authors declare no competing financial interests

Ultimately, embedding curcumin into combination and precision medicine strategies may substantially enhance its clinical utility in neurodegenerative disease management

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