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By employing the safe and feasible gene-editing tool CRISPR/Cas9 to engineer T cells, this method can mitigate exhaustion-inducing signaling pathways, preserve antitumor activity, and increase the in vivo persistence of CAR-T cells, thereby further overcoming limitations in cancer therapy (Fig

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For example, targeting carnitine palmitoyl transferase 1A and protein arginine methyltransferase 5 boosts immunotherapy by activating ACSL4 and inhibiting NRF2 [Figure 2] [54,137]