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The PW and control bars differed in colour, with the PW products being darker, but not obviously purple, such that participants could not know which treatment group they were allocated to

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doi: 10.1001/jamadermatol.2018.5040 194

You may need imaging exams to rule out other conditions that cause lower leg pain, like Achilles tendon ruptures or deep vein thrombosis (DVT)

Mount coverslip with a drop of glycerinum